Tuesday, March 22, 2011

Repeat Testing on Pipe "C" at Site #8 - March 19, 2011

4-Pole Creek at moderate level of 1.5 to 2', turbid water. Only Site #5 (under bridge) was tested along with Pipe "C", because at overall site #8, the site #1 (upstream control) has been nearly identical with the downstream site #5 (under bridge).  The object in this experiment was to continue to track the coliforms from Pipe "C" because the numbers seem to be inconsistent. On some days they are lower than the creek itself, and more recently, they are significantly higher. There is also the inconsistent appearance of suds. When these samples were collected (early in the day) there was abundant suds, similar to one of the previous test days.  We didn't have the camera when we took the samples, and the picture shown here was taken later in the day, and there were no suds at that time.  

                            # colonies                                   Coliforms/dl 
                      
Pipe "C"   1ml        4 (blue)                                         (400)

Pipe "C" 10ml     TNTC - blue dye                                N/A
                           destroyed by TNTC,
                           can't estimate

Site # 5     1ml        2 (blue)                                        (200)

Site # 5   10ml       55                                                  550

We can conclude that the Pipe C has more coliform cfu's than the stream itself on this date, but our numbers on the Pipe C, 1ml and Site # 5, 1ml have statistically inaccurate #'s of colonies (4 and 2 respectively).
The numbers of colonies on the 10ml analyses are much higher for Pipe C, but the blue dye was destroyed by there being too many colonies.  All we can say is that the coliform counts in Pipe C were much higher than in the creek itself.

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