Gram Stain Procedure

This was a gram stain procedure I did at Marshall with my dad around the dates February 4-7. He showed me how and what to do. It was interesting. Now I know how to do a gram stain procedure.


Here's what we did and the results:


Performed Gram stain on one G+ organism (Staphylococcus)

and one G- organism (E. coli).  Purpose - identification

1. Prepared smear using aseptic technique.
2. Air dried, heat fixed (gently).
3. Crystal Violet (10 sec.)
4. Iodine (1 min.)
5. Decolorizer (15sec,)
6. Safranin (1 min.)

Results: Staphylococcus appeared as purple spherical clumps, which was expected. The Escherichia coli was a faint pink bacillus, which was also expected.