Here's what we did and the results:
Performed Gram stain on one G+ organism (Staphylococcus)
and one G- organism (E. coli). Purpose - identification
- Prepared smear using aseptic technique.
- Air dried, heat fixed (gently).
- Crystal Violet (10 sec.)
- Iodine (1 min.)
- Decolorizer (15sec,)
- Safranin (1 min.)
Results: Staphylococcus appeared as purple spherical clumps, which was expected. The Escherichia coli was a faint pink bacillus, which was also expected.
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